This assay aids in selecting promising vaccines by demonstrating whether the vaccine-induced antibodies drive efficient complement deposition and subsequent opsonophagocytic killing. This assay utilizes polymorphonuclear leukocytes (PMNs), complement, test antibody and an antigen target. The antigen target for this assay is pneumococcal vaccine grade polysaccharide conjugated to a fluorescent polystyrene bead.
The interaction of antibody with antigen leads to the activation of the complement cascade. The activation of the complement cascade converts C1 to C3b, a protein that serves as an opsonin when bound to an antigen’s surface. Opsonization mediates phagocytosis, and the antigen (fluorescent bead) will be consumed and internalized by the phagocyte (PMN). The cells are then analyzed using a Flow Cytometer, and fluorescent signal is measured. This fluorescent signal is used to calculate a titer.
This service provides: instrumentation, scientist/analyst, bulk reagents, disposable supplies, raw data analysis, and biohazard control & disposal. Custom reporting, as well as sample and data archiving is available upon request.
|Assay Type||Opsonophagocytic Antibody Assay|
|Detection Method||Flow Cytometric|
|Specimen Type||Human Specimen|
|Notes||For in vitro research use only, not for diagnostic or therapeutic use.|