The serotyping assay detects the presence of pneumococcal carriage by use of nasopharyngeal swabs.
Components of the assay utilizes populations of polystyrene beads conjugated with a unique pneumococcal polysaccharide (PnPs), a proprietary anti-pneumococcal human monoclonal antibody cocktail, and a phycoerythrin conjugated anti-human reporter antibody.
In this assay, lysates are prepared from NP swabs. The lysates are mixed with the human anti-pneumococcal monoclonal antibody (mAb) cocktail.
In the absence of free PnPs from the sample lysate (no carriage) the mAbs bind to their target polysaccharide (bead). Upon addition of the reporter antibody, beads with bound monoclonal antibodies exhibit maximum fluorescence.
In the presence of free PnPs from the sample lysate (carriage) the monoclonal antibodies are competitively inhibited and are prevented from binding to their target bead. Upon addition of the reporter antibody, the beads with decreased bound monoclonal antibodies exhibit a measurable loss of fluorescent signal. The loss of fluorescent signal indicates the presence pneumococcal antigen and carriage in the nasopharynx.
This service provides: instrumentation, scientist/analyst, bulk reagents, disposable supplies, raw data analysis, and biohazard control & disposal. Custom reporting, as well as sample and data archiving is available upon request.
|Assay Type||Competitive Inhibition|
|Detection Method||Flow Cytometric|
|Specimen Type||Human Nasopharyngeal (NP) Swabs|
|Notes||For in vitro research use only, not for diagnostic or therapeutic use.|