The anti-pneumococcal IgG quantification assay detects and quantifies immunoglobulin G and/or immunoglobulin M in human serum samples. Briefly, this assay utilizes polystyrene beads conjugated with pneumococcal polysaccharides. There are 23 beads, each conjugated to a unique serotype (PNEUMOVAX23®). The beads/antigens will bind to the immunoglobulins in the serum sample(s). After binding occurs, all unbound antibodies are washed away. Next, a phycoerythrin conjugated antibody is added to the system. This reporter antibody will bind to the IgG/IgM antibodies. All unbound reporter antibody is washed away. The beads are then analyzed using a cytometric platform, and fluorescent signal is measured. Fluorescent signal is directly proportional to concentration. Concentration is determined by using a standard curve.
This service provides: instrumentation, scientist/analyst, bulk reagents, disposable supplies, raw data analysis, and biohazard control & disposal. Custom reporting as well as sample and data archiving is available upon request. Assay specific specialty reagents are invoiced at cost +15% handling fee.
|Assay Type||Immunoassay, Ligand binding, 23 Valent|
|Detection Method||Flow Cytometric|
|Specimen Type||Human Serum|
|Notes||For in vitro research use only, not for diagnostic or therapeutic use.|