The Flow Cytometric Anti-pneumococcal IgG Quantification Kit (FX3003) detects and quantifies immunoglobulin G in human serum samples. Provided in this kit are fluorescent polystyrene beads conjugated with pneumococcal polysaccharide. There are 23 beads, each conjugated to a unique serotype (PNEUMOVAX23®). The IgG Kit also provides an anti-pneumococcal mAb antibody standard and a phycoerythrin conjugated anti-human IgG reporter antibody. This kit comes with 2 positive controls.
In this assay, the immunoglobulin G antibodies from the patient samples will bind to the pneumococcal polysaccharide conjugated beads. After binding occurs, all unbound antibodies are washed away. Next, a phycoerythrin conjugated antibody is added to the system. The reporter antibody will bind to the IgG antibodies. All unbound reporter antibody is washed away. The beads are then analyzed using a cytometric platform. Fluorescent signal is directly proportional to concentration.
|Delivery Time||All kits are made on demand. Delivery time subject to availability. f (x) staff will send notification.|
|Assay Type||Immunoassay, Ligand Binding, 23 Valent|
|Detection Method||Flow Cytometer|
|Assay Time||3h 30m|
|Specimen Type||Human Serum|
|Storage Instructions||Store at 5°C|